DNA extraction method for screening yeast clones by PCR.
نویسندگان
چکیده
A simple procedure for isolating yeast DNA suitable for use as a template for PCR amplification is described. SDS treatment alone is sufficient for extraction of chromosomal DNA from yeast cells. Cells of a yeast colony are suspended in a small volume (about 20 microL) of a 0.25% SDS solution, mixed vigorously and centrifuged. The supernatant can be directly used as a template after dilution to give an SDS concentration of less than 0.01% in the final PCR mixture.
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ورودعنوان ژورنال:
- BioTechniques
دوره 28 4 شماره
صفحات -
تاریخ انتشار 2000